The following steps outline how to extract cheek cells for DNA analysis, such as PCR, according to the provided reference:
Steps for Cheek Cell Extraction
Here's a detailed breakdown of the process:
- Collect Cheek Cells: Gently scrape the inside of your cheek with a toothpick. This action dislodges cheek cells, which are then collected on the toothpick.
- Transfer to Buffer: Swirl the toothpick in extraction buffer. The extraction buffer helps to lyse the cells and release the DNA.
- Heat to Lyse: Heat the mixture for 10 minutes at 95 ℃. This further breaks down the cells, releasing the DNA.
- Use for PCR: The resulting DNA extract is now ready for use in PCR (Polymerase Chain Reaction).
This method is a simple and effective way to obtain DNA from cheek cells for downstream applications.