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How to extract cheek cells?

Published in Cell Extraction 1 min read

The following steps outline how to extract cheek cells for DNA analysis, such as PCR, according to the provided reference:

Steps for Cheek Cell Extraction

Here's a detailed breakdown of the process:

  1. Collect Cheek Cells: Gently scrape the inside of your cheek with a toothpick. This action dislodges cheek cells, which are then collected on the toothpick.
  2. Transfer to Buffer: Swirl the toothpick in extraction buffer. The extraction buffer helps to lyse the cells and release the DNA.
  3. Heat to Lyse: Heat the mixture for 10 minutes at 95 ℃. This further breaks down the cells, releasing the DNA.
  4. Use for PCR: The resulting DNA extract is now ready for use in PCR (Polymerase Chain Reaction).

This method is a simple and effective way to obtain DNA from cheek cells for downstream applications.