CS-Cells are cells that have undergone chromosome shredding through the use of a CRISPR-Cas12 DNA device, designed for efficient and safe molecular biomanufacturing.
Understanding CS-Cells
The term "CS-Cells" as described in the provided reference refers to a specific application of CRISPR-Cas12 technology to modify cells. Here's a breakdown:
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CRISPR-Cas12: This is a gene editing technology. CRISPR-Cas12 differs from the more commonly known CRISPR-Cas9. Cas12 is an enzyme that, guided by a CRISPR RNA (crRNA), cuts DNA.
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Chromosome Shredding: This is the outcome of using the CRISPR-Cas12 system in a specific way. Multiple cuts are made across the cell's chromosomes, effectively "shredding" them.
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Molecular Biomanufacturing: The ultimate goal is to use these CS-Cells for the production of biological molecules in a safer way. By shredding the chromosomes, the risk of uncontrolled cell growth (like in cancer) is theoretically reduced.
How CS-Cells are Created:
- Delivery of CRISPR-Cas12 System: The components of the CRISPR-Cas12 system (Cas12 enzyme and guide RNAs) are introduced into cells.
- Targeted DNA Cutting: The guide RNAs direct the Cas12 enzyme to specific locations on the chromosomes.
- Chromosome Fragmentation: Cas12 makes cuts at these targeted locations, leading to widespread fragmentation or "shredding" of the chromosomes.
Purpose of CS-Cells
- Enhanced Safety: A key advantage is the enhanced safety profile for biomanufacturing. By inducing chromosome shredding, the cells are rendered less likely to proliferate uncontrollably, reducing the risk of contamination or safety issues during production.
- Efficient Biomanufacturing: CS-Cells aim to provide an efficient platform for producing desired molecules in a controlled environment.
In summary, CS-Cells represent a novel approach to cell engineering using CRISPR-Cas12 technology to create cells with fragmented chromosomes for safer and more efficient molecular biomanufacturing.
