The last English queen to be executed was Anne Boleyn.
Anne Boleyn was the second wife of King Henry VIII and Queen of England from 1533 to 1536. Her marriage to Henry VIII, and her subsequent execution, were pivotal events in the English Reformation. Anne was initially a lady-in-waiting to Henry's first wife, Catherine of Aragon. Henry's desire to annul his marriage to Catherine, largely driven by his desire for a male heir, led to a break with the Roman Catholic Church and the establishment of the Church of England.
Anne's tenure as queen was relatively short. She was arrested in May 1536 on charges of treason, incest, and adultery. While the evidence against her was likely fabricated, she was found guilty and sentenced to death.
She was executed by beheading on May 19, 1536, at the Tower of London. Her execution was ordered by her husband, King Henry VIII.