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How to test for monkeypox?

Published in Mpox Diagnostic Testing 2 mins read

Testing for mpox (monkeypox) primarily involves real-time Polymerase Chain Reaction (PCR) tests, which are highly effective for diagnosis. This method is the standard for detecting the virus.


How Mpox is Diagnosed

The most common and reliable method for diagnosing mpox is through real-time PCR testing. This advanced molecular test identifies the genetic material of the mpox virus, confirming the presence of an active infection.

Specimen Collection for Mpox Testing

For accurate diagnosis, proper specimen collection is crucial.

  • Type of Specimen: Clinicians primarily collect samples by swabbing lesions (skin sores or blisters) that are characteristic of mpox.
  • Quantity: It is recommended that clinicians collect two swabs from each lesion. This practice ensures that sufficient material is available, not only for initial testing but also for any potential additional analyses, such as clade-specific testing, if required.

Where to Get Tested for Mpox

Mpox testing is widely accessible through various public health and commercial channels:

  • Public Health Departments: Testing services are available through local, state, territorial, and tribal health departments.
  • Commercial Laboratories: Many large commercial laboratories also offer mpox testing, making it accessible through healthcare providers.

Key Aspects of Mpox Testing

To summarize the essential components of mpox testing:

Aspect Details
Primary Method Real-time Polymerase Chain Reaction (PCR)
Specimen Type Swabs collected directly from mpox lesions (e.g., rashes, blisters, pustules).
Collection Protocol Clinicians should collect two separate swabs from each lesion. This dual collection allows for robust primary testing and provides a backup sample for advanced analysis, such as determining the specific viral clade (lineage) if necessary.
Test Availability Testing is widely available through various channels, including:
  • Local, state, territorial, and tribal health departments
  • Many large commercial laboratories
Patients with suspected symptoms should consult their healthcare provider, who can facilitate testing.

For more detailed information on mpox diagnosis and testing, healthcare professionals can refer to resources from reputable organizations like the Centers for Disease Control and Prevention (CDC).