Yes, guard cells do have mitochondria.
Guard cells, which regulate the opening and closing of stomata in plants, require energy to function. According to the provided reference, guard cells possess both bio-energetically active chloroplasts and mitochondria to meet their energy demands. The observation that guard cells have a relatively high number of mitochondria compared to chloroplasts suggests that oxidative phosphorylation, a process that occurs in the mitochondria, is the primary energy source for stomatal opening.
In summary, guard cells contain mitochondria that play a crucial role in providing the energy needed for stomatal function.
