While none of the four main Golden Girls characters were born male, The Golden Girls featured a guest character named Gil Kessler, who was revealed to be transgender. Gil was born Anna Maria Bonaduce.
The character Gil appears in the Season 7 episode "You Gotta Have Hope." Sophia falls for Gil, but hesitates due to a vague feeling something is amiss. Gil reveals his transgender identity, explaining he always felt like "a great man trying to get out."
Therefore, while not a main "Golden Girl," Gil Kessler is the character connected to the premise of being born male (and transitioning).
